Remove the washing solution by gentle tapping.

Rinse the test sample container and transfer the rinse water to the flask:

0.25 Change from Baseline in FEV1 (L)

Hold eyelids open to ensure complete contact with water.

This buffer is used for diluting antisera and washing IF slides

This also applies to solutions used to flush butterfly and other devices.

Fill the wash-bottle in the gas-line (nitrogen or air) two-thirds full with ethyl acetate.

Rinse the weighing vessel with distilled water and transfer the rinse water to the volumetric flask.

In paragraph 5.2 it may be more effective to use hot water in place of cold water to wash the residue after filtration.

If this problem persists, a fifth wash may be introduced at the extraction stage, shaking vigorously for 30 seconds.

However, the assessment of leaching in soils with pH below 6 deserves further attention, since, for soils with pH below 6, leaching at concentration levels exceeding 0,1 >ISO_7>i>ISO_1>g/l may occur in some realistic vulnerable situations.

Rinse the round-bottomed flask with 20 ml of hydrochlorid acid (3.17) and use this to wash the resin column.

Carefully wash the crucible, adaptor and filter flask with 150-200 ml water, and return the rinsing water to the beaker used for the precipitation.

Then transfer the sample solution quantitatively to a 25 ml volumetric flask, using about 5 ml of water for rinsing.

51 * Quinolin-8-ol and bis (8-hydroxyquinolinium) sulphate * Stabilizer for hydrogen peroxide in rinse-off hair-care preparations * 0,3 % calculated as base " * * *

WHEREVER MENTION IS MADE OF WATER FOR DISSOLUTION , DILUTION OR WASHING PURPOSES , DISTILLED WATER , OR DEMINERALIZED WATER OF AT LEAST EQUIVALENT PURITY SHALL BE USED.

All operations should be carried out in the absence of light (using amber glassware, or glassware protected with aluminium foil) and oxygen (flush with nitrogen).

Their use must be followed by thorough rinsing of such instruments and working equipment with potable water except where the directions for use of such substances render such rinsing unnecessary.

Repeat this rinsing operation two or three times.

Where washing is carried out with liquids applied under pressure and following the disinfection, re-contamination of the previously cleansed or disinfected parts must be avoided.

The third wash can be shaken vigorously for 30 seconds.

Percolate 30-ml n-hexane to remove any impurities.

Any operation involving preparation of solutions, dilution, rinsing or washing, mentioned in the methods of analysis without indication as to the nature of the solvent or diluent employed, implies that water must be used.

After washing with cold water further spraying with disinfectant should be applied.

This may result from inadequate washing at the extraction stage.

After filtering and washing, the precipitate is dried at 250 oC and weighed.

After washing, dry the filter and ash at 550 oC in a platinum crucible.

Any non-ionic surfactants remain in the effluent. Soap fatty acids are separated by extraction with ethanol containing CO2.

Unbound conjugate is removed by washing and chromogen-containing substrate is added.

If ethanol is added it is essential to carry out a further two vigorous water washes.

After washing, 50 μl of guinea-pig antiserum to the antigen used in point 4 is added to each well.

If there are no traces of fat on the surface of the dish, quantitatively transfer, during the fourth washing, as much as possible of the sediment into the crucible.

After rinsing with 50 ml of distilled water, transfer the resin to a beaker, add 50 ml of the 4 M solution of acetic acid (3.1.1.3) and stir for five minutes.

In its opinion, the Committee agreed that sufficient information was available to reliably estimate the leaching behaviour of the substance for soils with pH above 6.

Grease and other dirt should be removed from all surfaces by soaking with a degreasing agent and then washing with water under pressure.

The model calculations of the leaching behaviour of isoxaflutole and its degradation products were subsequently revised along the lines suggested by the Scientific Committee.

After washing three times with PBS the infected cells are overlaid with the relevant medium containing 1 % w/v agar and either 0,01 mg/ml trypsin or no trypsin.

Place the tube in a water bath set at 30 oC exactly and keep there for exactly thirty minutes. Immediately add 10 ml of 0.1 N hydrochloric acid (3.1), cool rapidly to 20 oC and transfer the contents of the tube quantitatively to a titration vessel, rinsing twice with 5 ml of water.

In a second consultation on the same substances the Scientific Committee was asked to comment on the appropriate degradation kinetics to be assumed in model calculations of the leaching behaviour.

Take up the ash with 10 ml of 10 % sulphuric acid (4.5) and transfer it with washings of distilled water (4.1) to a 50 ml volumetric flask (5.10), and fill to the mark at room temperature with distilled water (4.1).

Collagen must be produced by a process ensuring that unprocessed category 3 material is subjected to a treatment involving washing, pH adjustment using acid or alkali followed by one or more rinses, filtration and extrusion.

Stir the solution using a magnetic stirrer (3.2.16), add a few drops of bromocresol purple (3.2.5) and add the diluted ammonia solution (3.2.9) until the colour turns violet (the solution is weakly acid from the residue of acetic acid used for rinsing).

Stir the solution using a magnetic stirrer (3.2.16), add a few drops of bromocresol purple (3.2.5) and add the dilute ammonia solution (3.2.9) until the colour turns violet (the solution is initially weakly acid from the residue of acetic acid used for rinsing).

Subheading 1509 10 covers only olive oils defined in Sections I and II obtained solely using mechanical or other physical means under conditions, and particularly thermal conditions, that do not lead to deterioration of the oil, and which have undergone no treatment other than washing, decantation, centrifugation or filtration.

After radiolabelling the antibody and diluting a 10 µl sample with 1.5 ml saline, immediately determine the radiochemical purity by Instant Thin Layer Chromatography on silica gel impregnated glass fiber strips, 1 × 9 cm using acetone as the solvent.

In the event of accidental contact, remove cream by rinsing with water. • Do not apply the cream internally. • Do not use more cream than your doctor has advised. • Do not cover the treated area with bandages or other dressings after you have applied Aldara cream. • If the treated site becomes too uncomfortable, wash the cream off with mild soap and water.

DNA extraction and purification has to be accomplished by either using conventional procedures(3), ready-to-use kits (based on the principle: solubilisation of the meat sample in lysis buffer which contain surfactants, detergents and proteinase K, application of the solubilised sample to a DNA-binding resin, removal of non-DNA compounds by repeated washing steps, and finally elution of purified DNA in water or low-salt buffer), or extracting the DNA in sodium hydroxide solution.