NMR parameters:

Some typical educational NMR experiments that demonstrate the essential features are presented as well.

A miniature nuclear magnetic Resonance (NMR) spectrometer designed for educational purposes is described.

The possibility of characterization and quantification of rat liver damage in vivo by NMR relaxation times was studied.

SNIF-NMR:

Distillation of wine far SNIF-NMR

Result of deuterium isotope correlations of ethanol measured by NMR:

This is carried out by distilling wine as described in point 3.1 of method No 8 (SNIF-NMR).

3A201.b. does not control magnets specially designed for and exported 'as parts of medical nuclear magnetic resonance (NMR) imaging systems.

"Detecting enrichment of grape musts, concentrated grape musts, rectified concentrated grape musts and wines by application of nuclear magnetic resonance of deuterium (SNIF-NMR)."

The information on carbon 13 content combined with that obtained from SNIF-NMR enables the added quantities of mixtures of sugars or alcohols derived from C3 and C4 plants to be determined.

The results show that under satisfactory conditions, and in particular those for measurement using SNIF-NMR, the different distillation systems do not produce significant variation in the determination of the >ISO_7>ä13>ISO_1>C value of wine ethanol.

NMR spectrometer fitted with a specific 'deuterium' probe tuned to the characteristic frequency Vo of the field Bo (e.g. for Bo = 7,05 T, Vo = 46,05 MHz and for Bo = 9,4 T, Vo = 61,4 MHz) having a proton decoupling channel (B2) and field-frequency stabilization channel (lock) at the fluorine frequency.

10 mm diameter NMR probe:

Spectral data (UV, IR, NMR)

Conditions for obtaining NMR spectra

Spectral data (UV, IR, NMR or mass spectrum)

Preparation of alcohol sample for NMR measurement

The conditions for obtaining NMR spectra are as follows:

RECORDING OF 2H NMR SPECTRA OF THE ALCOHOL AND THE WATER

Spectra (UV/VIS, IR, NMR, MS), molecular extinction at relevant wavelengths

For each of the 10 spectra (see NMR spectrum for ethanol, Figure 2a), determine:

Absorption spectra (UV/VIS, IR, NMR, MS), molecular extinction at relevant wavelengths (;)

Figure 2a2H NMR spectrum of an ethanol from wine with an internal standard (TMU: N, N-tetramethylurea)

When the isotope ratio of water is determined by NMR from the water-TMU mixture, the following relationship is used:

Preparation of a water sample for the NMR measurement, for the purpose of a possible determination of its isotope ratio

Absorption spectra (UV/VIS, IR, NMR), and a mass spectrum, molar extinction at relevant wavelengths, where relevant (5)

Optional software (e.g. SNIF-NMR) suitable for the spectrometer computer enables such calculations to be carried out on-line.

15 mm diameter NMR probe:in a previously weighed bottle, collect 7 ml alcohol obtained as in 3.1.2 and weigh it to the nearest 0,1 mg (mA);

3A201.b. does not control magnets specially designed for and exported 'as parts of' medical nuclear magnetic resonance (NMR) imaging systems.

If the laboratory has a mass spectrometer for determining isotope ratios, the measurement may be carried out on this instrument to reduce the load on the NMR spectrometer.

Authentication of a must or of a wine may be checked by the method using NMR of deuterium described for detecting the enrichment of musts, rectified concentrated musts and wines.

'Detecting enrichment of grape musts, concentrated grape musts, rectified concentrated grape musts and wines by application of nuclear magnetic resonance of deuterium (SNIF-NMR).'

DETECTING ENRICHMENT OF GRAPE MUSTS, CONCENTRATED GRAPE MUSTS, RECTIFIED CONCENTRATED GRAPE MUSTS AND WINES BY APPLICATION OF NUCLEAR MAGNETIC RESONANCE OF DEUTERIUM (SNIF-NMR/RMN-FINS)

The European Commission granted a marketing authorisation valid throughout the European Union for Helicobacter Test INFAI to INFAI, Institut für biomedizinische Analytik & NMR-Imaging GmbH on 14 August 1997.

Ultraviolet/Visible (UV/VIS), infrared (R), nuclear magnetic resonance (NMR), and mass spectra (MS) of purified active substance and molecular extinction at relevant wavelengths, must be determined and reported.

The UV/visible absorption spectra, IR, NMR and MS spectra, where necessary for the identification of the impurities considered to be of toxicological, ecotoxicological or environmental significance must be determined and reported.

The results show that under satisfactory conditions, and in particular those for measurement using SNIF-NMR, the different distillation systems do not produce significant variation in the determination of the δ13C value of wine ethanol.

This procedure enables between 98 and 98,5 % of the total alcohol in the wine to be recovered in a distillate with a strength of between 92 and 93 % mas (95 % vol), a strength for which the NMR conditions described in section 4 have been established.