From the mean peak height (area) produced by injection of the sample solution (5.3.3) determine the concentration of lasalocid sodium g/ml) by reference to the calibration graph.

Measure the height and the width at half height of the acetonitrile and chloroform peaks and calculate the area of both peaks, using the formula: height × width at half height.

The peak width, at half height, must be within ± 10 % of the original peak width produced by the unfortified sample extract.

Inject each calibration solution (3.10.3) several times and determine the mean peak heights (areas) for each concentration.

Plot a calibration graph using the mean peak heights (areas) as the ordinates and the corresponding concentrations in µg/ml as the abscissae.

Only the height of the lasalocid sodium peak should be enhanced to an extent based on the amount of lasalocid sodium added and the dilution of the extract.

Detector, flame ionization: adjust its sensitivity so that when 3 l of solution 4.7 is injected, the height of the acetonitrile peak is about three quarters full-scale deflection.

Inject the sample extracts obtained in 5.2.1 or 5.2.2 several times, using the same volume as taken for the calibration solution and determine the mean peak heights (areas) of the lasalocid sodium peaks.

Check the stability of the chromatographic system, injecting the calibration solution (3.10.3) containing 4,0 µg/ml several times, until constant peak heights (or areas) and retention times are achieved.

Calculate the ratios of the peak heights of the investigated preservatives to the peak height of the internal standard.

In this case only one peak shall be obtained, the enhanced peak height (or area) being equivalent to the amount of added analyte.

From the chromatograms obtained determine the ratios of the peak heights of the standard preservative solutions to the peak height of the internal standard.

R= Peak area or height of congener / Peak area or height of IS

Plot a graph for each preservative relating the peak height ratio to the concentration of each standard solution.

Response factor = Peak area or height IS / Peak area or height congener ×

Response factor = Peak area or height IS / Peak area or height congener Ă-

Peak area or height congener / Peak area or height IS × MIS (g) / MSAMPLE (g)

Peak area or height congener / Peak area or height IS Ă- MIS (g) / MSAMPLE (g)

Use the calibration curve (6.2.2) and the ratios of the peak heights of the investigated preservatives to the peak height of the internal standard to calculate the concentration of the preservatives in the sample solution.

Record the chromatogram and measure the peak heights.

W1 and W2 = peak widths at half height, in milimetres,

W1 and W2 = peak widths at half height, in millimetres,

W1 and Wa = peak widths at half height (in millimetres),

W1 and W2 = peak widths at half height (in millimetres),

Measure either peak areas or peak heights for congener and internal standard peaks.

y = the height or area of peaks in any single determination,

y = the height or area of peaks in any single determination;

Plot the maximum peak height values ascertained for the four 4-toluenesulphonamide spots against the corresponding quantities of 4-toluenesulphonamide (i.e. 4, 6, 8, 10 tig of 4-toluenesulphonamide per spot).

The nearest peak maximum in the chromatogram shall be separated from the designated analyte peak by at least one full peak width at 10 % of the maximum height of the analyte peak.

Measure the area (or height) of the peaks relating to the VC and internal standard, if used.

Measure the area (or height) of the peaks relating to the VC and to the internal standard if used.

If not, determine the area under each peak by multiplying the height of the peak by its width at mid-height, and where necessary take into account the various attenuations used during the recording.

This is produced by plotting peak heights as a function of the concentration of formaldehyde standard:

Construct a graph in which the ordinate value shows the areas (or heights) of the VC peaks or the ratio of the areas (or heights) of VC peaks to the areas (or heights) of the internal standard peaks and the abscissa values show the quantities of VC added (mg) relating to the quantities of the sample of foodstuff weighed in each phial (kg).

For each solution determine the ratios of the peak heights of the investigated preservatives to the height of the internal standard peak obtained from the chromatograms.

A temperature programmed gas chromatograph fitted with a flame ionisation detector and integrator or other data handling system capable of measuring peak areas or peak heights.

Construct a graph in which the ordinate values show the areas (or heights) of the VC peaks of the duplicate solutions or the ratio of these areas (or heights) to those of the relevant internal standard peaks and the abscissa values show the VC concentrations of the duplicate solutions.

Construct a graph in which the ordinate values show the areas (or heights) of the VC peaks of the duplicate solutions, or the ratio of these areas (or heights) to those of the relevant internal standard peaks, and the abscissa values show the VC concentrations of the duplicate solutions.

No broad signals due to the presence of organic materials shall be seen in the voltamograms.Inorganic matrix constituents may influence peak heights in DPASV.

From the integrator peak areas or peak heights for each injection calculate the ratio R for each congener and plot a graph of R versus the concentration ratio of congener to internal standard (IS), C. A linear plot should be obtained, with a correlation coefficient of at least 0,99.

With gas chromatography (GC) or liquid chromatography (LC), the peak width at half-maximum height shall be within the 90-110 % range of the original width, and the retention times shall be identical within a margin of 5 %.

Use the ratios of the heights of the peaks of the investigated preservatives to the height of the 2-methoxybenzoic acid (internal standard) peak and the calibration graph to calculate the concentration of the acid preservatives in the sample solution.

R= trans-anethole peak height or area divided by the estragole peak height or area.

This enables quantification by peak height.

Integrator capable of peak height measurement.

The integrator (6.40.6) automatically calculates the peak height H of the GMP peak.

hd,s= peak height (area) of diclazuril in the sample solution (5.2.1)

hd,s= peak height (area) of diclazuril in the sample solution (5.2.2)

Calculations of the results may also be done by peak height measurement.

hd,c= peak height (area) of diclazuril in the calibration solution (3.10)