m = amount of aflatoxin B1 in ng represented by the B1 peak of the sample, calculated as follows m = P (sample) P (st1) + P (st2) × 2 r (st) P (sample) = peak area of aflatoxin B1 for the sample P (st1) = peak area of aflatoxin B1 resulting from the preceeding injection of reference standard (3.13.3) P (st2) = peak area of aflatoxin B1 resulting from the following injection of reference standard (3.13.3) r (st) = injected amount of aflatoxin B1 in the reference standard (3.13.3) in ng Vm = volume of the injected sample extract in ml Ve x t = final volume of sample extract in ml, allowing for any dilution that was made (5.3) M = mass of sample in g Vf = volume of filtrate transferred to Florisil cartridge (5.4.1.2) in ml Vc = volume of chloroform, used for the extraction of the sample in ml If the procedure is followed as in this protocol, the formula reduces to:
m m = P + P × 2 r st P (vzorec) = površina vrha aflatoksina B1 vzorca P(st1) = površina vrha aflatoksina B1, ki izhaja iz predhodnega vbrizganja referenčnega standarda (3.13.3) P(st2) = površina vrha aflatoksina B1, ki izhaja iz naslednjega vbrizganja referenčnega standarda (3.13.3) r(st) = vbrizgana količina aflatoksina B1 v referenčnem standardu (3.13.3) v ng Vm = prostornina vbrizganega ekstrakta vzorca v ml Vext = končna prostornina ekstrakta vzorca v ml, z upoštevanjem opravljene razredčitve (5.3) M = masa vzorca v g Vf = prostornina filtrata, prenesenega v kartušo Florisil (5.4.1.2) v ml Vc = prostornina kloroforma, uporabljenega za ekstrahiranje vzorca v ml Če upoštevamo postopek v tem protokolu, se formula glasi: