Odtehtamo 10 g vzorca na 5 mg natančno, postavimo v 500-mililitrsko bučko z ravnim dnom in dodamo 2 g fino mlete bele gorčice (3.1) (kot vir encimov) in 200 ml vode, ki ima temperaturo 20 °C.
Weigh 10 g of the sample to the nearest mg, place in a 500 ml flat-bottomed flask and add 2 g of finely ground white mustard (3.1) (an enzyme source) and 200 ml of water at 20 ĹźC.