Reject the eluate.

Evaporate the eluate to dryness using a steam bath or in a rotary evaporator.

Reject the eluate.

Evaporate the eluate to dryness on a steam bath or in a rotary evaporator.

Discard the eluate.

Discard the effluent.

Collect the effluent in a beaker.

Collect the effluent in a 100 ml volumetric flask.

Evaporate the eluate to dryness using a steam bath or in a rotary evaporator.

Dry the eluted product in a nitrogen flow and dissolve in isopropanol or actone (5 ml).

Collect the effluent in a 100 ml volumetric flask, and rinse the column with water until about 90 ml of the effluent is obtained.

Concentrate the eluate on the rotary evaporator at 40 °C to 50 °C until no more acetone is distilled.

The eluate is evaporated and the residue redissolved in a specific volume of chloroform or of a mixture of benzene and acetonitrile.

Evaporate the latter almost to dryness at a temperature not exceeding 50 oC and under a stream of inert gas (3.11) with the rotary evaporator (4.5).

The first 100 ml of the chlorobenzene eluate contains the 2-aminoazobenzene.

Passing about 5000-6000 ml of ammonium bicarbonate solution (5.3.4) down the column at a flow rate of approximately 10 ml/min until the eluate is free from anionic surfactants (methylene blue test) regenerates the anion-exchange resin.

Passing an additional quantity of ammonium bicarbonate solution (4.3.5) down the column at a flow rate of approximately 10 ml/min until the eluate is free from anionic surfactants (methylene blue test) regenerates the anion-exchange resin.

for radiopharmaceuticals, additional detailed instructions for extemporaneous preparation and quality control of such preparation and, where appropriate, maximum storage time during which any intermediate preparation such as an eluate or the ready-to-use pharmaceutical will conform with its specifications.

Wash the column with 0,3 M ammonium bicarbonate solution (4.3.5) until free of chloride.

Wash the column with 0,3 M ammonium bicarbonate solution (5.3.4) until free of chloride.

Wash the column with deionised water at a rate of 10-30 ml/min until the eluate is free of chloride.

Using the eluting solvent (3.14) develop for at least 20 minutes in a tank saturated with solvent vapour, until the pinkcoloured hydrazone zone is clearly separated.

Eluting solvent for thin layer chromatography: mix 75 ml diethyl ether a.p., 25 ml ethyl acetate a.p. and 4 70 ml 96 % (w/v) acetic acid a.p. Renew after 2 to 3 chromatographies.

Evaporate the collected aqueous phases (6.2.2.2) or the eluate (6.2.2.3) almost to dryness in the rotary evaporator under reduced pressure.

The first 10 ml of eluate is further treated as described in 6.2.2.4

Successive eluate fractions should be collected and analyzed by the chosen method.

The water solubility is determined when the mass concentration of the eluate is constant.

The anion-exchange resin is regenerated by passing an additional quantity of ammonium bicarbonate solution (2. 3. 5) down the column at a flow rate of approximately 10 ml/min until the eluate is free from anionic surfactants (methylene blue test).

Wash the column with deionized water at a rate of 10-30 ml/min until the eluate is free of chloride.

For generator-eluates, tests for mother radio-nuclides and for other constituents of the generator system shall be provided.

Those fractions from the middle eluate range where the concentrations are constant (± 30 %) in at least five consecutive fractions are used to determine the solubility in water.

As soon as the whole of the mixture has been transferred into the column, elute with hydrochloric acid (4.13) in such a way that 10 ml of eluate is obtained in approximately 10 minutes (if necessary, this elution can be performed under a slight nitrogen pressure).

Transfer the eluate obtained in (5.4.2) into a 1 litre separating funnel.

Wash the column with distilled water until the effluent is neutral.

Wash the column until the effluent is neutral.

Discard the eluates.

Then elute with 150 ml of the chloroform/methanol mixture (3.7) and collect the whole of the eluate.

reservoir of eluent, inert gas, degassing of the eluent, composition of the eluent, impurities,

Add 0,5 g calcium carbonate and 1 ml saturated lead acetate solution to the effluent.

Elute the aflatoxin B1 with 50 ml water/acetone mixture (3.5.3), and collect the whole of the eluate in a 50 ml volumetric flask.

Transfer the extract (5.2) quantitatively to the top of the prepared Amberlite column (5.3.1) and elute, discarding the eluate.

Stirring all the time, add 2,5 ml of saturated lead acetate solution (3.1.1.6) and 0,5 g of calcium carbonate (3.1.1.7) to the effluent:

Add the prepared eluate into the shielded vial, shake and allow the labelling reaction to proceed for ten minutes.

Elute aflatoxin B1 with 40 ml of the acetone-water mixture (3.5.1) and collect the whole of the eluate in the round bottomed flask of the rotary evaporator (4.4).

Elute the robenidine from the column using 100 ml methanol (3.1), at a flow rate of 2 to 3 ml/minute and collect the eluate in a 250 ml round bottomed flask.

Leave the remaining methanol for 10 minutes to equilibrate with the resin and continue the elution at a rate not exceeding 20 ml/min, collecting the eluate in the same round-bottomed flask.

The first 10 ml of eluate is further treated as described in 6.2.2.4.

The anion-exchange resin is regenerated by passing about 5 000-6 000 ml of ammonium bicarbonate solution (2.3.4) down the column at a flow rate of approximately 10 ml/min until the eluate is free from anionic surfactants (methylene blue test).

Passing about 5000-6000 ml of ammonium bicarbonate solution (5.3.4) down the column at a flow rate of approximately 10 ml/min until the eluate is free from anionic surfactants (methylene blue test) regenerates the anion-exchange resin.

qualitative and quantitative particulars of the eluate or the sublimate.