Positive control

+ve control

A positive control for the activity of one metabolizing system for all strains is 2-aminoanthracene.

Positive control (C++, C-):

+ve cont = positive control.

In control experiments famotidine had positive inotropic and positive chronotropic effects and decreased Q.

medium alone (positive control)

Row 3 A - H is the positive control.

Where, NC is the OD of the negative control and PC the OD of positive control.

control using a positive serum of sensitivity at the start of the reaction;

The test is valid when the optical density (OD) of negative control (NC) is higher than 1,0 and the OD of positive control (PC) is lower than 0,2.

The end-point titre for the positive control based on 50 % PI should be 1 in 240 (within a range from 1 in 120 to 1 in 480).

One of the main strategies of this control system is the linking of the positive self-image with the existing negative attitude, in our example, the negative attitude towards Jews.

if sentinel chickens show positive or inconclusive serological results the imported birds must be subjected to virological examination.

However, if the positive control serum titre is greater than 1 in 480 and the test samples are still negative then the negative test samples can be accepted.

Serum samples to be tested, and positive and negative control sera, are diluted 1 in 25 in PBS + 5 % (w/v) skimmed milk + 0,05 % (v/v) Tween 20, 100 >ISO_7>Ä›>ISO_1>l per well.

S erum samples to be tested, and positive and negative control sera, are diluted 1 in 25 in PBS + 5 % (w/v) skimmed milk + 0,05 % (v/v) Tween 20, 100 >ISO_7>ě>ISO_1>l per well.

For titration, make a twofold dilution series from 1 in 25 (100 >ISO_7>Ä›>ISO_1>l/well), one serum per plate column, and do the same with positive and negative controls.

F or titration, make a twofold dilution series from 1 in 25 (100 >ISO_7>ě>ISO_1>l /well), one serum per plate column, and do the same with positive and negative controls.

description of the laboratory samples (e.g. size, quality, date of sampling), positive and negative controls as well as reference material, plasmids and alike used,

Titrate the positive control sera in a twofold dilution series, from 1 in 5 to 1 in 640, across column 1 in blocking buffer (PBS containing 0,05 % (v/v) Tween-20, 5,0 % (w/v) skimmed-milk powder (Cadbury' s MarvelTM) and 1 % (v/v) adult bovine serum) to give a final volume of 50 >ISO_7>ě>ISO_1>l/well.

A positive finding for avian influenza or Newcastle disease or the confirmation of these diseases in the quarantined birds or the sentinel chickens shall not be reported as an outbreak within the context of Council Directive 82/894/EEC(3) on the notification of animal diseases within the Community, but should nevertheless be reported to the Commission.

By way of derogation from paragraph 4 the competent authority may decide that after positive findings for Newcastle disease in one or more birds or in the sentinel chickens that the birds need not be destroyed, if, at least 30 days after the death or clinical recovery of the last case, sampling according to Annex C(1)(B) (ignoring the reference to the time period specified) has been carried out with negative results.

- ISAV-infected SHK-1 cell culture or other susceptible cell culture (positive control).

Positive control:

V.4.2. If available, a kidney imprint from an ISAV-infected Atlantic salmon is recommended as an additional positive control.

Known positive control serum

Apply the FISH procedure (van Beuningen et al, 1995) with the OLI-1 PCR primer (Seal et al, 1993) The culture must show the same reaction as the positive control.

A compound known to produce chromosomal aberrations in vivo is employed as a positive control and a negative (solvent) control group is also included in the design of each experiment.

Check the positive control slide first.

Use of negative and positive controls

Reference or fortified material containing known amounts of analyte, at or near either the maximum permitted limit or the decision limit (non-compliant control sample) as well as compliant control materials and reagent blanks should preferably be carried through the entire procedure simultaneously with each batch of test samples analysed.

Reference or fortified material containing known amounts of analyte, at or near either the permitted limit or the decision limit (non-compliant control sample) as well as compliant control materials and reagent blanks should preferably be carried through the entire procedure simultaneously with each batch of test samples analysed.

Check against the DNA marker and against the positive control.

Use of negative and positive control:

In each experiment a positive control is included;

The profile of the culture must be identical to that of the positive control.

Positive and negative control sera should be used in the test.

For the positive control group, a single sampling time is sufficient.

Positive controls have to be conducted also for two purposes:

The IF titre of the culture must be equivalent to that of the positive control.

Proper negative and positive controls have to be included in the test.

Pulse centrifuge the vials with the boiled sample and positive control.

Positive controls/reference substances shall be included where necessary.

The ELISA value of the culture must be equivalent to that of the positive control.

Prepare an inoculum of 106 cells per ml from the culture and a positive control strain.

For a test to be valid the positive controls must score positive and the negative controls must score negative.

Both positive and negative (solvent) controls are to be used in each experiment.

Separate the positive control plants from the other plants to avoid cross-contamination.

Add, in the specified order, 2- 5 µ1 of the sample(s), water control and positive control to the vials with the PCR reaction mix.