Rinsing solutions

Fill the wash-bottle in the gas-line (nitrogen or air) two-thirds full with ethyl acetate.

Liquid for washing.

Filtering and washing

After filtering and washing, the precipitate is dried at 250 ĹźC and weighed.

Af ter filtering and washing, the precipitate is dried at 250 şC and weighed.

After washing, dry the filte r and ash at 550 şC in a platinum crucible.

Any non-ionic surfactants remain in the effluent. Soap fatty acids are separated by extraction with ethanol containing CO2.

After washing, dry the filter and ash at 550 ĹźC in a platinum crucible.

The anionic surfactants are then obtained as ammonium salts, by elution with an aqueous isopropanolic solution of ammonium bicarbonate.

livestock density shall be defined taking into account all grazing livestock kept on the farm or, in the case of a commitment to limit nutrient leaching, all animals kept on the farm which are relevant to the commitment in question.

P lace the tube in a water bath set at 30 şC exactly and keep there for exactly thirty minutes. Immediately add 10 ml of 0.1 N hydrochloric acid (3.1), cool rapidly to 20 şC and transfer the contents of the tube qua ntitatively to a titration vessel, rinsing twice with 5 ml of water.

Place the tube in a water bath set at 30 ĹźC exactly and keep there for exactly thirty minutes. Immediately add 10 ml of 0.1 N hydrochloric acid (3.1), cool rapidly to 20 ĹźC and transfer the contents of the tube quantitatively to a titration vessel, rinsing twice with 5 ml of water.

Collagen must be produced by a process ensuring that unprocessed category 3 material is subjected to a treatment involving washing, pH adjustment using acid or alkali followed by one or more rinses, filtration and extrusion.

Stir the solution using a magnetic stirrer (3.2.16), add a few drops of bromocresol purple (3.2.5) and add the dilute ammonia solution (3.2.9) until the colour turns violet (the solution is initially weakly acid from the residue of acetic acid used for rinsing).

Cleaning of float and measuring cylinder

This buffer is used for washing the slides.

Filter under vacuum into the weighed crucible, rinse the beaker with the liquid for washing (4.7), wash the precipitate three times with the liquid for washing (60 ml in all of the liquid for washing) and twice with 5 to 10 ml of water.

Repeat this operation three more times, using the light petroleum to rinse the inside of the neck of the vessel.

Where the system is not used for more than two days, this flushing must be followed by flushing with methanol (5.2.7).

If the extracted matter is not wholly soluble in the light petroleum, or in case of doubt, extract the fat completely from the vessel by repeatedly washing with warm light petroleum.

Wherever mention is made to water for solution, dilution or rinsing purposes, distilled water, deionized water or demineralized water of at least equivalent purity, shall be used unless otherwise specified.

after washing with cold water, further spraying with disinfectant must be applied,

Soap fatty acids are separated by elution with ethanol containing CO2.

other raw material is subjected to a treatment with acid or alkali, followed by one or more rinses.

After a last rinse, bound enzyme is visualised using appropriate ELISA substrates (OPD or others).

Collect the precipitate by rinsing the absorbers with water (3.10) and wash the crucible with 50 ml of the same water.

Biuret can be purified beforehand by washing with an ammoniacal solution (10 %), then with acetone and drying in a vacuum.

Following another rinse as above, HRP-conjugated streptavidin is added and allowed to react for one hour at 20 °C.

Following another rinse as above, HRP-conjugated streptavidin is added and allowed to react for one hour at 20 °C.

Transfer the contents of the flask quantitatively into a 500 ml separating funnel using several rinses of distilled water, amounting in all to about 50 ml.

A complex combination of hydrocarbons produced by the distillation of a cut from the catalytic cracking process, having undergone an alkaline washing.

Clean tubes and screw-caps by leaching several hours in perchloric acid (4.11), followed by five subsequent rinsings with water (4.2), and finally dry at 100 °C.

Clean tubes and screw-caps by leaching several hours in perchloric acid (4.11), followed by five subsequent rinsings with water (4.2), and finally dry at 100 °C.

This must be done so as not to abnormally purge or abnormally load the evaporative control devices fitted to the vehicle.

For some substances shown to be irritating by this test, additional tests using rabbits with eyes washed soon after instillation of the substance may be indicated.

The anionic surfactants are then obtained as ammonium salts, by elution with an aqueous isopropanolic solution of ammonium bicarbonate.

(8) The report on iprodione and further information were also submitted to the Scientific Committee on Plants for separate consultation. The Scientific Committee was asked to comment on the predicted environmental concentrations in soil and groundwater, and on the acceptable operator exposure level proposed by the rapporteur. In its opinion, the Committee agreed that sufficient information was available to reliably estimate the leaching behaviour of the substance for soils with pH above 6. However, the assessment of leaching in soils with pH below 6 deser ves further attention, since, for soils with pH below 6, leaching at concentration levels exceeding 0,1 μg/l may occur in some realistic vulnerable situations. The Committee saw no need to use an additional safety factor for the derivation of the acceptabl e operator exposure level. The observations of the Scientific Committee were taken into account in this Directive and the review report.

livestock density shall be defined taking into account all grazing livestock kept on the farm or, in the case of a commitment to limit nutrient leaching, all animals kept on the farm which are relevant to the commitment in question.

Following rinsing with PBS-Tween-20 buffer, biotinylated antibodies of a specificity corresponding to that of the coating antibodies are added and allowed to react for 60 minutes at 20 °C.

Following rinsing with PBS-Tween-20 buffer, biotinylated antibodies of a specificity corresponding to that of the coating antibodies are added and allowed to react for 60 minutes at 20 °C.

After rinsing of wells with PBS-Tween-20 buffer, the virus to be identified is added to the wells in two-or four-fold dilution steps and allowed to react with the coating antibody for 60 minutes at 37 °C.

After rinsing of wells with PBS-Tween-20 buffer, the virus to be identified is added to the wells in two-or four-fold dilution steps and allowed to react with the coating antibody for 60 minutes at 37 °C.

Carry out a third extraction by again repeating the operations described in A.1.5.3. to A.1.5.8. using 15 ml of the diethyl ether and 15 ml of the light petroleum and rinsing the long inner limb of the fitting as described in A.15.10.

produced by a production process which involves appropriate measures to minimise contamination of hides and skins, preparation of the hides and skins by brining, liming and intensive washing followed by exposure of the material to a pH of > 11 for > three hours at temperature > 80 °C and followed by heat treatment at >140 °C for 30 minutes at > 3,6 bar or by an equivalent production process approved by the Commission after consultation of the appropriate Scientific Committee;

produced by a production process which involves appropriate measures to minimise contamination of hides and skins, preparation of the hides and skins by brining, liming and intensive washing followed by exposure of the material to a pH of > 11 for > three hours at temperature > 80 °C and followed by heat treatment at >140 °C for 30 minutes at > 3,6 bar or by an equivalent production process approved by the Commission after consultation of the appropriate Scientific Committee;

Washout Phase

INH and INH washout N=158, 155, 143, 139, 123.

Deionized water shall be used to rinse the equipment.

Alternately, the flushing may be performed by infusion.