not less than 1 x103 CCID50* not less than 12,5 x103 CCID50* not less than 1 x103 CCID50*

1 ml 1: cell culture infective dose 50 % 2: egg infective dose 50 %

methods for maintenance of cell culture, if applicable,

A cell culture study should be performed in human cell or tissue cultures of different organs.

Cidofovir Inhibition of Virus Multiplication in Cell Culture

An increased 3H-thymidine incorporation in the erythroid nucleated spleen cells has been found in vitro (mouse spleen cell culture) after incubation with erythropoietin. It could be shown with the aid of cell cultures of human bone marrow cells that erythropoietin stimulates erythropoiesis specifically and does not affect leucopoiesis.

If human cell or tissue cultures of specific organs are not available, other mammal cell and tissue cultures can be used.

A variety of cell lines, strains or primary cell cultures, including human cells, may be used

These strains may be specifically identified using the DIF test on the sediments of the cell cultures or by PCR.

In the in vitro mammalian cell gene mutation test, cultures of established cell lines or cell strains can be used.

The history of animal or cell passage material should be provided.

Cell cultures are exposed to the test substance both with and without metabolic activation.

Appropriate cell cultures should be in constant readiness for confirming a negative diagnosis.

No differences were observed between the cell-culture and the comparator egg-derived vaccine.

Cytarabine is cytotoxic to a wide variety of proliferating mammalian cells in culture.

2 produced in yeast cells (Saccharomyces cerevisiae) by recombinant DNA technology 3

* produced in Chinese Hamster Ovary cells (CHO) by recombinant DNA technology

au *** produced in Saccharomyces cerevisiae cells by recombinant DNA technology

Cell cultures are treated with Colcemid® or colchicine usually for 1-3 hours prior to harvesting.

The ratio between inoculum size and volume of cell culture medium should be about 1:10.

produced in yeast cells (Saccharomyces cerevisiae) by recombinant DNA technology.

produced in yeast cells (Saccharomyces cerevisiae) by recombinant DNA

The other ingredients in the vaccine are: sodium chloride, water for injections.

for viruses and viroids, cell culture studies using purified infective virus and primary cell cultures of mammalian, avian and fish cells

The in vitro chromosome aberration test may employ cultures of established cells lines, cell strains or primary cell cultures.

Autolysed samples can be cytotoxic to the cell culture and consequently of limited use.

The active ingredient of ORENCIA, abatacept, is a protein produced in cell cultures.

It is particularly important that cell culture conditions ensure a cell cycle time within the normal historical range of the cells or cell line used.

1 ml 1: cell culture infective dose 50 % 2: egg infective dose 50 % For a full list of excipients, see section 6.1.

Two further cell culture passages may be necessary in order to detect small amounts of the virus in the sample.

Additionally, live measles vaccine and live mumps vaccine are produced in chick embryo cell culture.

Tissue culture studies suggest that cell growth in human breast tumours may be stimulated by prolactin.

The requirements laid down in table 1 must be fulfilled in any laboratory where the ASF virus is to be amplified by replication in cell cultures.

Prepare a cell suspension of 1 × 1055 cells/ml in culture medium and dispense 100 µl culture medium only into the peripheral wells of a 96-well tissue culture microtiter plate (= blanks).

Lamivudine in combination with zidovudine exhibits synergistic anti-HIV activity against clinical isolates in cell culture.

a microscopic examination and culture test for trichomonas foetus on a sample of vaginal washings or preputial washings;

Lamivudine has been shown to be highly synergistic with zidovudine, inhibiting the replication of HIV in cell culture.

produced in yeast cells (Saccharomyces cerevisiae) by recombinant DNA technology 3 adsorbed on aluminium phosphate (AlPO4) 4 propagated in VERO cells

From each virus supernatant-serum mixture at least two cell cultures are inoculated with 50 μl each and then incubated at 15 °C.

Alternatively aliquots of 10 % of the medium constituting the primary culture is inoculated directly into a well with fresh cell culture (well to well subcultivation).

It could be shown with the aid of cell cultures of human bone marrow cells that epoetin alfa stimulates erythropoiesis specifically and does not affect leucopoiesis.

3 produced in Saccharomyces cerevisae cells by recombinant DNA technology rod

Pretitrated virus also diluted in serum-free culture medium and containing 100 TCID50/0,05 ml is then added to each well.

* 50 % cell culture infectious dose 1 produced in chick embryo cells.

Atazanavir has activity against HIV-1 Group M subtype viruses A, B, C, D, AE, AG, F, G, and J isolates in cell culture.

isolation and identification of ISAV in cell culture from at least one sample from any fish on the farm as described in Part III,

isolation and identification of ISAV in cell culture from a single sample from any fish on the farm as described in Part III;

Virus isolation is based on the inoculation of sample material on susceptible primary cell cultures of porcine origin, monocytes and macrophages cells.

However, foot-and-mouth disease virus isolates with a prolonged history of transmission between pigs may also preferentially grow in porcine cell culture systems.

virus infectivity assay, (ii) serum toxicity controls, (iii) uninoculated cell culture controls, (iv) reference antisera.