Cell culture medium with at least 10 % serum should be used.

Cell culture study

Cell Culture Infective Dose 50 %

Cell culture medium shall be removed from each well, including those of positive and negative controls, and placed in labelled sterile tubes.

Overall, 41 samples from the urethra, cervix and urine were tested with cell culture, DIF and PCR methods.

Male urethral specimens and cervical specimens were tested by cell culture and male urethral specimens also by DFA staining.

The commercially available polymerase chain reaction (PCR) based assay, Amplicor Chlamydia Trachomatis Test, for the detection of Chlamydia trachomatis DNA in patients with urogenital infections was compared with cell culture of Clamydia trachomatis and direct fluorescent antibody (DFA) assay.

Another molecule isolated from saliva that was found to block HIV-1 infection of mononuclear cells in culture was identified as secretory leukocyte protease ihibitor (SLPI).

Celebrating the importance of cultural diversity for the full realization of human rights and fundamental freedoms proclaimed in the Universal Declaration of Human Rights and other universally recognized instruments,

The paper reviews briefly the major advances in cancer immunotherapy, including various immunisation techniques, treatment with cytokines and their analogues, and the use of monoclonal antibodies and immune cells cultured in vitro.

Autolysed samples can be cytotoxic to the cell culture and consequently limit its use.

Two further cell culture passages may be necessary in order that a small amount of virus in the sample is detected.

However, at present all commercial ELISA are less sensitive than the virus isolation on cell culture and their sensitivity is significantly better on blood samples from piglets than from adult pigs.

After incubation the mixture is inoculated onto cell cultures which are incubated for three to five days.

Virus isolation is based on the incubation of sample material on susceptible cell cultures of porcine origin.

From the economic perspective, newspresenters serve the same purpose as celebrities in other spheres of cultural production:

Coat ELISA plates with AHSV antigen extracted from infected cell cultures and diluted in carbonate/bicarbonate buffer, pH 9,6.

The morphology and ATP levels of cultured epithelial cells exposed to serum were better maintained than those exposed to hypromellose.

After this incubation period the cultures are fixed and any viral replication in the infected cells is detected by an immune labelling system.

The results of the VNT are expressed as the reciprocal of the initial serum dilution at which half the inoculated cell cultures (50 % end point) fail to show viral replication (no specific labelling).

In the laboratory, cultured human corneal epithelial cells were exposed to serum drops and hypromellose, and their viability evaluated with fluorescent viability staining (Calcein AM ethidium homodimer) and an ATP assay.

A celebrity is a 'locus of formative social power in consumer capitalism allowing for the configuration, positioning, and proliferation of certain discourses about the individual and individuality in contemporary culture' says David Marshall.

These other areas should be available for the preparation of glassware and media, the maintenance and preparation of non-infected cell cultures, the processing of sera and serological testing (other than methods using live classical swine fever virus), and the provision of administrative and clerical support.

The television newspresenters circulate between fashion competitions, openings and parties (The Slovene Woman of the Year Contest, On the Sunny Side of the Alps, Fashion Jana Selects the Best-dressed Man in Slovenia; Fashion Thoughts of the Best-dressed Men and Women in Slovenia, etc.), and mix with celebrities from other spheres of the local popular culture.

Today - when newspresenters appear on hoarding posters; when they are turning into commercial objects, trademarks, and local celebrities; when they live their secondary lives in the social chronicles of tabloids; when they compete for best-dressed local celebrity; when they expose minor secrets about the private lives of the local showbiz in their articles; and when the news has finally become part of local popular culture - this is more true than ever.

- uninfected SHK-1 cell culture or other susceptible cell culture (negative control),

- ISAV-infected SHK-1 cell culture or other susceptible cell culture (positive control).

Batches of virus in low cell culture passage numbers are propagated in cell culture flasks on BF-2 or RTG-2 cells for VHSV and on EPC or FHM cells for IHNV.

For each dilution and each cell line a minimum of about 2 cm2 cell area, corresponding to one well in a 24-well cell culture tray, has to be utilised.

Demonstration of the agent in body tissues or fluids or isolation in animals or cell culture

III.1). The ratio between inoculum size and volume of cell culture medium should be about 1:10.

Cell cultures and media

Use of cell culture trays is recommended, but other units of similar or bigger growth area are acceptable as well.

- number of cell cultures;

Incubation of cell cultures

Inoculation of cell cultures

(b) isolation and identification of ISAV in cell culture from a single sample from any fish on the farm as described in Part III;

(i) isolation and identification of ISAV in cell culture from at least one sample from any fish on the farm as described in Part III,

The different steps of the manufacturing process such as organ/tissue dissociation, selection of the cell population of interest, in vitro cell culture, cell transformation either by physico-chemical agents or gene transfer shall be documented.

III.1. Cell cultures and media

Isolation (in cell culture or in a laboratory animal) of rabies virus from saliva, cerebrospinal fluid (CSF), or central nervous system tissue

If evidence of CPE has been observed in a cell culture, medium (supernatant) is collected and examined by one or more of the following techniques:

III.4.1. Cell culture medium shall be removed from each well, including those of positive and negative controls, and placed in labelled sterile tubes.

III.3. Incubation of cell cultures

III.2. Inoculation of cell cultures

Precipitating antigen is prepared in any cell culture system that supports the rapid multiplication of the appropriate serotype(s) of epizootic haemorrhagic disease virus.

If the colour of the cell culture medium changes from red to yellow indicating medium acidification, pH adjustment with sterile bicarbonate solution or equivalent substances has to be performed to ensure cell susceptibility to virus infection.

Cultures of established cell lines as well as primary cell cultures may be used.

At total CPE, virus is harvested by centrifugation of cell culture supernatant at 2000 x g for 15 minutes, filter sterilised through 0,45 ěm membrane filter and distributed in labelled cryotubes.

At total CPE, virus is harvested by centrifugation of cell culture supernatant at 2000 x g for 15 minutes, filter sterilised through 0,45 ěm membrane filter and distributed in labelled cryotubes.