Tissue culture studies suggest that cell growth in human breast tumours may be stimulated by prolactin.

Tissue culture medium (e.g. Eagle's MEM) containing 0,04M Hepes buffer, 0,01 % bovine serum albumin and antibiotics, pH 7,2.

Since chlamydia can be propagated in tissue culture it is possible to study their biological, epidemiological and other properties.

Considering moreover the ethical, psychological and socio-cultural problems inherent in the transplantation of organs and tissues;

cut trees retaining foliage, - plant tissue cultures.

TCID50 Tissue culture infective dose at the 50 %end point

IV.1.4. PCR confirmation of isolation of ISAV in tissue culture

plant tissue cultures.

seeds, tissue cultures

(vii) plant tissue cultures.

3-17-A3 (supplied as hybridoma tissue-culture supernatant) stored at -20 °C or freeze-dried, diluted 1/50 with blocking buffer before use, directed against the group-specific polypeptide p7.

3-17-A3 (supplied as hybridoma tissue-culture supernatant) stored at -20 °C or freeze-dried, diluted 1/50 with blocking buffer before use, directed against the group-specific polypeptide p7.

in cooperation with the suppliers of antigen and the reference laboratory for the identification of the foot-and-mouth disease virus, to review strains stored in the Community reserves and to assist in selecting suitable new strains to be adapted to tissue culture for future vaccine production;

Demonstration of the agent in body tissues or fluids or isolation in animals or cell culture

These techniques must always be accompanied by inoculation of non-fixed tissue material on cell cultures.

(b) seedling or tissue cultures obtained in vitro, in solid or liquid media, transported in sterile containers;

(b) seedling or tissue cultures obtained in vitro, in solid or liquid media, transported in sterile containers, and

Isolation (in cell culture or in a laboratory animal) of rabies virus from saliva, cerebrospinal fluid (CSF), or central nervous system tissue

Either prepare a smear of the ooze or the suspended tissue on a microscope slide or prepare a smear of a 48 hour culture on YPGA or SPA (Appendix 1).

Cell cultures to be used for inoculation with tissue material should be young (4 to 48 hours old) and actively growing (not confluent) at inoculation.

This section describes the procedures required for PCR amplification of part of segment 8 of the ISAV genome that may be carried out on fish tissue or ISAV in culture

For all of the following Annex A species, tissue cultures obtained in vitro, in solid or liquid media, transported in sterile containers, are not subject to the provisions of this Regulation)

Antiserum-treated organ suspension shall be inoculated into young actively growing cell cultures to give a final dilution of tissue material to culture medium of 1:1000 For each organ suspension 40 >ISO_7>ě>ISO_1>l of inoculum shall be added to one well containing 2 ml of culture medium.

It involves a rapid screening test, isolation of the pathogen from infected vascular tissue on diagnostic media and, in case of a positive result, identification of the culture as Ralstonia solanacearum.

Where practical difficulties arise (e.g. bad weather conditions, non-working days, laboratory problems, etc.) which make it impossible to inoculate cells within 48 hours after the collection of the tissue samples it is acceptable to freeze the tissue specimens in cell culture medium at - 20 °C or below and carry out virological examination within 14 days.

Where practical difficulties arise (e.g. bad weather conditions, non-working days, laboratory problems, etc.) which make it impossible to inoculate cells within 48 hours after the collection of the tissue samples it is acceptable to freeze the tissue specimens in cell culture medium at - 20 °C or below and carry out virological examination within 14 days.

The different steps of the manufacturing process such as organ/tissue dissociation, selection of the cell population of interest, in vitro cell culture, cell transformation either by physico-chemical agents or gene transfer shall be documented.

Antibiotic-treated organ suspension is inoculated into cell cultures in two dilutions, i.e. the primary dilution and, in addition, a 1:10 dilution thereof, resulting in final dilutions of tissue material in cell culture medium of 1:100 and 1:1000, respectively, (in order to prevent homologous interference).

Where practical difficulties arise (e.g. incubator breakdown, problems with cell cultures, etc.) which make it impossible to inoculate cells within 48 hours after the collection of the tissue samples, it is acceptable to freeze the supernatant at -80 °C and carry out virological examination within 14 days.

Where practical difficulties arise (e.g. incubator breakdown, problems with cell cultures, etc.) which make it impossible to inoculate cells within 48 hours after the collection of the tissue samples, it is acceptable to freeze the supernatant at -80 °C and carry out virological examination within 14 days.

The requirements laid down in table 1 must be fulfilled in any laboratory where the ASF virus is to be amplified by replication in cell cultures. However, post-mortem examinations, processing of tissues for DIFT or PCR and serology using inactivated antigens, may be carried out at a lower containment level, provided that the minimum requirements of table 1 are fulfilled, basic hygiene is used and post-operational disinfection with safe disposal of carcases, tissues and sera are carried out.

III.5.3. If bacterial contamination occurs in the primary culture, the test must be set up again using the tissue homogenate stored at - 80 °C. Prior to inoculation the tissue homogenate is centrifuged at 4000 x g for 30 minutes at 0 to 6 °C and the supernatant is filtered at 0,22 >ISO_7>ě>ISO_1>m. If bacterial contamination occurs during the subcultivation step the supernatant shall be filtered at 0,22 >ISO_7>ě>ISO_1>m, inoculated onto fresh cells and incubated for a further 14 to 18 days.

As none of the species or higher taxa of flora included in Annex A is annotated to the effect that its hybrids shall be treated in accordance with the provisions of Article 4.1 of the Regulation, this means that artifically propagated hybrids produced from one or more of these species or taxa may be traded with a certificate of artificial propagation, and that seeds and pollen (including pollinia ), cut flowers, seedling or tissue cultures obtained in vitro, in solid or liquid media, transported in sterile containers of these hybrids are not subject to the provisions of the Regulation.

PCR confirmation of isolation of ISAV in tissue culture

Prepare a cell suspension of 1 × 1055 cells/ml in culture medium and dispense 100 µl culture medium only into the peripheral wells of a 96-well tissue culture microtiter plate (= blanks).

- increases bone formation in bone tissue culture as well as osteoblast precursor replication and

“ Tissue culture studies suggest that cell growth in human breast tumours may be stimulated by prolactin.

In addition, tissue culture studies suggest that cell growth in human breast tumours may be stimulated by prolactin.

Tissue-culture grown viral antigens are trapped by a rabbit hyperimmune anti-serum to swine vesicular disease adsorbed to the solid phase.

The test is carried out in flat-bottomed tissue culture grade microtitre plates using susceptible cells such as IB-RS-2, BHK-21 or calf kidney cells.

The VN test must be considered as the reference test, but has the disadvantage that it takes 2 to 3 days to complete and requires tissue culture facilities.

Human immunoglobulin anti-vaccinia shall contain not less than 500 IU per ml of vaccinia antibody as determined by a neutralization test on chorio-allantoic membranes or in tissue culture.

3-17-A3 (supplied as hybridoma tissue-culture supernatant) directed against the group-specific polypeptide VP7, stored at -20 °C or freeze-dried and diluted 1/100 with blocking buffer before use.

The quantitative VN micro-test for swine vesicular disease virus antibody detection is performed with IB-RS-2 cells or an equivalent cell system in flat-bottomed tissue culture grade microtitre plates.

If human cell or tissue cultures of specific organs are not available, other mammal cell and tissue cultures can be used.

If the quantity and quality of samples from vesicular lesions submitted for examination is insufficient for immediate examination by ELISA, the growth of virus in tissue culture will be necessary to amplify viral antigen.

National Laboratories shall ensure that the tissue culture systems in use for foot-and-mouth virus isolation are sensitive to the full range of serotypes and strains for which the laboratory maintains a diagnostic capacity.

Negative cultures must be inoculated on fresh tissue cultures at 48 or 72 hours and this blind passage examined up to 72 hours later.

The simultaneous inoculation of a porcine cell line and one of the above-mentioned tissue culture systems (preferably primary bovine thyroid cells) is a useful guide as to whether vesicular samples contain swine vesicular disease virus or foot and mouth disease virus, as the former will only grow in cells of porcine origin.

A cell culture study should be performed in human cell or tissue cultures of different organs.